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fume 音标拼音: [fj'um] n. 臭气,烟,激怒
vt. 熏
vi. 冒烟 臭气,烟,激怒熏冒烟 fume n 1: a cloud of fine particles suspended in a gas [ synonym: { smoke}, { fume}] v 1: be mad, angry, or furious 2: emit a cloud of fine particles; " The chimney was fuming" [ synonym: { fume}, { smoke}] 3: treat with fumes, expose to fumes, especially with the aim of disinfecting or eradicating pests [ synonym: { fumigate}, { fume}] 4: be wet with sweat or blood, as of one' s face [ synonym: { reek}, { fume}] Fume \ Fume\ ( f[= u] m), n. [ L. fumus; akin to Skr. dh[= u] ma smoke, dh[= u] to shake, fan a flame, cf. Gr. qy` ein to sacrifice, storm, rage, qy` mon, qy` mos, thyme, and perh. to E. dust: cf. OF. fum smoke, F. fum[' e] e. Cf. { Dust}, n., { Femerell}, { Thyme}.] 1. Exhalation; volatile matter ( esp. noxious vapor or smoke) ascending in a dense body; smoke; vapor; reek; as, the fumes of tobacco. [ 1913 Webster] The fumes of new shorn hay. -- T. Warton. [ 1913 Webster] The fumes of undigested wine. -- Dryden. [ 1913 Webster] 2. Rage or excitement which deprives the mind of self- control; as, the fumes of passion. -- South. [ 1913 Webster] 3. Anything vaporlike, unsubstantial, or airy; idle conceit; vain imagination. [ 1913 Webster] A show of fumes and fancies. -- Bacon. [ 1913 Webster] 4. The incense of praise; inordinate flattery. [ 1913 Webster] To smother him with fumes and eulogies. -- Burton. [ 1913 Webster] 5. ( Metal.) Solid material deposited by condensation of fumes; as, lead fume ( a grayish powder chiefly lead sulphate). [ Webster 1913 Suppl.] { In a fume}, in ill temper, esp. from impatience. [ 1913 Webster]
Fume \ Fume\, v. t. 1. To expose to the action of fumes; to treat with vapors, smoke, etc.; as, to bleach straw by fuming it with sulphur; to fill with fumes, vapors, odors, etc., as a room. [ 1913 Webster] She fumed the temple with an odorous flame. -- Dryden. [ 1913 Webster] 2. To praise inordinately; to flatter. [ 1913 Webster] They demi- deify and fume him so. -- Cowper. [ 1913 Webster] 3. To throw off in vapor, or as in the form of vapor. [ 1913 Webster] The heat will fume away most of the scent. -- Montimer. [ 1913 Webster] How vicious hearts fume frenzy to the brain! -- Young. [ 1913 Webster]
Fume \ Fume\, v. i. [ imp. & p. p. { Fumed}; p. pr. & vb. n. { Fuming}.] [ Cf. F. fumer, L. fumare to smoke. See { Fume}, n.] 1. To smoke; to throw off fumes, as in combustion or chemical action; to rise up, as vapor. [ 1913 Webster] Where the golden altar fumed. -- Milton. [ 1913 Webster] Silenus lay, Whose constant cups lay fuming to his brain. -- Roscommon. [ 1913 Webster] 2. To be as in a mist; to be dulled and stupefied. [ 1913 Webster] Keep his brain fuming. -- Shak. [ 1913 Webster] 3. To pass off in fumes or vapors. [ 1913 Webster] Their parts are kept from fuming away by their fixity. -- Cheyne. [ 1913 Webster] 4. To be in a rage; to be hot with anger. [ 1913 Webster] He frets, he fumes, he stares, he stamps the ground. -- Dryden. [ 1913 Webster] While her mother did fret, and her father did fume. -- Sir W. Scott. [ 1913 Webster] { To fume away}, to give way to excitement and displeasure; to storm; also, to pass off in fumes. [ 1913 Webster] 285 Moby Thesaurus words for " fume": achromatize, ado, aerate, aerify, afterdamp, agitation, anhydrate, aroma, ash, ashes, atomize, be livid, be pissed, blackdamp, blanch, blast- freeze, bleach, blow, blow a gasket, blow up, bluster, bobbery, boil, boil over, boiling, brand, brawl, breath, breathe out, brine, bristle, broil, brouhaha, browned off, burn, bustle, cacophony, calx, carbon, carbonate, carry on, chafe, chaos, charcoal, chlorinate, chokedamp, churn, cinder, clinker, cloud, coal, coke, commotion, conturbation, cure, damp, decolor, decolorize, definite odor, dehydrate, desiccate, detectable odor, dim, discolor, discomposure, disorder, disquiet, disquietude, distill, disturbance, drain, drain of color, dross, dry, dry- cure, dry- salt, dudgeon, dull, ebullience, ebullition, eddy, effervescence, effluvium, emanation, embalm, embroilment, emit, essence, etherify, etherize, etiolate, evacuate, evaporate, excitement, exhalation, exhale, exhaust, expire, explode, fade, fanaticism, feery- fary, ferment, fermentation, fetid air, fever, feverishness, fidgets, firedamp, flap, flare up, flatus, flavor, flip out, fluid, fluidize, flurry, fluster, flutteration, foam, foment, fomentation, foofaraw, fractionate, fragrance, freeze, freeze- dry, frenzy, fret, fumes, fumigate, furor, furore, fury, fuss, gas, gasify, give off, give out, give vent to, go on, have a conniption, high dudgeon, hubbub, huff, hurly- burly, hurry, hurry- scurry, hydrogenate, inquietude, irradiate, jerk, jitters, jumpiness, kipper, lava, let out, maelstrom, malaise, malaria, marinade, marinate, mephitis, miasma, miff, moil, mummify, nerviness, nervosity, nervousness, odor, open the floodgates, open the sluices, oxygenate, pale, pandemonium, passion, pell- mell, perfume, peroxide, perturbation, pet, pickle, pique, pissed off, pollution, preservatize, puff, puff of smoke, quick- freeze, racket, rage, raise Cain, raise hell, raise the devil, raise the roof, rant, rant and rave, rave, redolence, reek, refrigerate, restlessness, roil, rout, row, ruckus, ruffle, rumpus, salt, savor, scent, scoria, season, seethe, seething, send out, simmer, sizzle, slag, smell, smog, smoke, smoke- cure, smolder, smoulder, smudge, smut, soot, spoor, spray, steam, stench, stew, stink, stir, storm, storminess, stuff, sublimate, sublime, subtle odor, sullage, sweat, swirl, swirling, swivet, take on, tarnish, tempestuousness, throw a fit, throw off, tiff, tizzy, to- do, tone down, trace, trail, trepidation, trepidity, tumult, tumultuation, tumultuousness, turbidity, turbulence, turmoil, twitter, unease, unrest, uproar, upset, vapor, vaporize, volatile, volatilize, vortex, wash out, water vapor, whiff, whirl, whiten, wildness, yeastiness, zeal, zealousness
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- MTT实验——原理、步骤、注意事项、示例图 - 知乎
二甲基亚砜 (DMSO)能溶解细胞中的甲臜，用 酶联免疫检测仪 在570nm波长处测定其光吸收值，可间接反映活细胞数量。 在一定细胞数范围内，MTT结晶形成的量与细胞数成正比。
- MTT比色法-细胞增殖及细胞活性测定-实验 - 百度文库
MTT实验原理：为活细胞线粒体中的琥珀酸脱氢酶能使外源性MTT还原为水不溶性的蓝紫色结晶甲瓒 (Formazan)并沉积在细胞中，而死细胞无此功能。 二甲基亚砜 (DMSO )能溶解细胞中的甲瓒，用酶标仪在490nm波长处测定其光吸收值，在一定细胞数范围内，MTT结晶形成的量与细胞数成正比。 根据测得的吸光度值 (OD值)，来判断活细胞数量，OD值越大，细胞活性越强。 4、弃MTT液，MTT加入培养4h后，结晶可充分形成。 将上清去掉，该过程要注意不能把Formazan结晶移走 [10]。 5、每孔加入150ulDMSO，摇床10min，使结晶物充分溶解。 在酶联免疫检测仪OD490nm处测量各孔的吸光值。
- MTT比色法-实验步骤、操作方法-丁香实验
但由于不同细胞贴壁后面积差异很大，因此，在进行MTT试验前，要进行预实验检测其贴壁率、倍增时间以及不同接种细胞数条件下的生长曲线，确定试验中每孔的接种细胞数和培养时间，以保证培养终止致细胞过满。
- 四唑盐法 (MTT)细胞活力和增殖检测方案 - Sigma-Aldrich
用于测定人白介素-6 (hIL-6)对7TD1细胞（鼠-鼠杂交瘤细胞，DSMZ，ACC 23）的增殖刺激作用（见图3）。 将7TD1细胞接种到微孔板（组织培养级，96孔，平底），接种密度2 × 10 3 cells 孔，100 μl培养基，其中含不同含量的IL-6【例如，终浓度0 1-10 U mL (0 001-0 1 ng mL)】。
- 细胞增殖检测——MTT法 - 知乎
MTT法又称MTT比色法，是一种常用的检测细胞存活和生长的方法。 检测原理：活细胞线粒体中的琥珀酸脱氢酶能使外源性MTT还原为不溶于水的蓝紫色结晶甲臜（Formazan）并沉积在细胞中，而死细胞无此功能。 二甲基亚砜（…
- MTT法_百度百科
MTT法又称MTT比色法，是一种检测细胞存活和生长的方法。 其检测原理为活细胞线粒体中的琥珀酸脱氢酶能使外源性MTT还原为水不溶性的蓝紫色结晶甲臜（Formazan）并沉积在细胞中，而死细胞无此功能。
- MTT法-实验步骤、操作方法-丁香实验
二甲基亚砜能溶解细胞中的紫色结晶物，用酶联免疫检测仪在490 nm 波长处测定其光吸收值，可间接反映细胞数量。 在一定细胞数范围内，MTT结晶物形成的量与细胞数成正比。
- MTT 检测方案 | Abcam
详细介绍用于测定细胞增殖或细胞毒性的 MTT 检测的试剂制备步骤和实验方案。 MTT 检测法是细胞生物学中用于评估细胞活性和增殖的基础方法。 它在药物发现、毒理学和癌症研究中特别有价值。 本实验方案简化了操作流程，指导研究人员完成试剂制备、孵育和吸光度测量。 检测原理基于活细胞中线粒体脱氢酶对 MTT 底物的酶促还原作用，产生可定量的比色信号。 只有活细胞才能将 MTT 转化为着色甲臜产品，从而将其与死细胞或非活细胞区分开来。 凭借其简单的工作流程和与高通量方式的兼容性，MTT 检测仍然是研究人员寻求有关细胞对治疗和环境压力源反应的准确和可重复的数据的首选。 MTT 检测利用黄色四氮唑盐 MTT，这种物质被活细胞中的线粒体酶还原后形成不溶性紫色甲臜晶体。
- MTT实验原理、方法、实验步骤 - 黄灿华教授 实验室
其检测原理为活细胞线粒体中的琥珀酸脱氢酶能使外源性MTT还原为水不溶性的蓝紫色结晶甲瓒（Formazan）并沉积在细胞中，而死细胞无此功能。 二甲基亚砜（DMSO）能溶解细胞中的甲瓒，用酶联免疫检测仪在490nm波长处测定其光吸收值，可间接反映活细胞数量。 在一定细胞数范围内，MTT结晶形成的量与细胞数成正比。 该方法已广泛用于一些生物活性因子的活性检测、大规模的抗肿瘤药物筛选、细胞毒性试验以及肿瘤放射敏感性测定等。 它的特点是灵敏度高、经济。 缺点：由于MTT经还原所产生的甲瓒产物不溶于水，需被溶解后才能检测。 这不仅使工作量增加，也会对实验结果的准确性产生影响，而且溶解甲的有机溶剂对实验者也有损害。 MTT 溶液的配制方法：通常，此法中的MTT 浓度为5mg ml。
- 2025年MTT实验方法步骤. pdf-原创力文档
1．介绍一种巨噬细胞杀伤活性的MTT检测：于24孔培养板中加入1×109 L腹腔巨噬细胞 (1ml 孔)，培养2h后洗去未贴壁细胞，加入不同浓度的MDP。 收集对数生长期的UMR106 细胞，调整细胞浓度至1×108 L，加入各孔，效靶比（E T）为10:1，再培养24h，充分振荡。
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